BIO Web Conf.
Volume 65, 2023EBWFF 2023 - International Scientific Conference Ecological and Biological Well-Being of Flora and Fauna (Part 2)
|Number of page(s)||8|
|Section||Preventive Healthcare and Urban Ecology|
|Published online||04 September 2023|
Genetic detection of folate pathway inhibitors and outer membrane porins genes among gentamicin-resistant klebsiella pneumoniae isolates
University of Kufa, Department of Biology, Faculty of Science, Kufa, Iraq
* Corresponding author: email@example.com
Infectious diseases are one of the leading causes of morbidity and mortality worldwide, and antibacterial resistance exacerbates the situation. So this study aimed to investigate genes responsible for folate inhibitor pathway antagonists and outer membrane protein genes among gentamicin-resistant K.pneumoniae isolates. The results showed that out of 481 specimens, 270 (56.13%) showed bacterial growth versus 211 (43.87%) showed no bacterial growth. According vitek-2 system recorded 94 isolates as K. pneumoniae. Data demonstrated that 42/94 (44.68%) K. pneumoniae isolates were resistant to gentamicin compared with 24/94(25.53%) and 28/94(29.78%) of isolates were intermediate and sensitive to this antibiotic respectively. Results of antibiotic susceptibility showed that the highest bacterial resistance was piperacillin 41/42 (97.7%). while netilmycin 16/42 (38%) had the least resistance. PCR amplification results showed that dfr-B, Dfr-G and Dfr-K were 13/42 (30.9%), 17/42 (40.4%) and 37/42 (88.0%)) respectively. While Dfr-A did not detect. Also PCR results showed that 39 (92.8%) and 32 (76.1%) of the isolates had Ompk35 and Ompk36 genes respectively.
© The Authors, published by EDP Sciences, 2023
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