Issue |
BIO Web Conf.
Volume 73, 2023
5th International Conference on Tropical Resources and Sustainable Sciences (CTReSS 5.0 2023)
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Article Number | 05028 | |
Number of page(s) | 6 | |
Section | Sustainability and Technology | |
DOI | https://doi.org/10.1051/bioconf/20237305028 | |
Published online | 08 November 2023 |
Effect of bean sprout on in vitro multiplication of Musa acuminata
1 Faculty of Agro-based Industry, Universiti Malaysia Kelantan Jeli Campus, Jeli, Kelantan
2 Institute of Food Security & Sustainable Agriculture (IFSSA), Universiti Malaysia Kelantan Jeli Campus, Jeli, Kelantan
3 Faculty of Earth Sciences, Universiti Malaysia Kelantan Jeli Campus, Jeli, Kelantan
* Corresponding author: suhana@umk.edu.my
Musa acuminata or Pisang Berangan is popular in Kelantan, but due to a lack of knowledge on modern farming technologies, many Musa acuminata are affected by diseases and are of low quality. Plant tissue culture has numerous advantages, including a rapid rate of multiplication, and the prevention of disease. Plant growth regulator such as Benzyl Amino Purine (BAP) is commonly used in media to grow banana explants, however, Murashige and Skoog (MS) media production with BAP hormone is expensive for low-income farmers. Therefore, this research had been done by substituting the BAP hormone by using bean sprout extract as an organic supplement for banana culture. The additives that were used in this project were non-centrifuged and centrifuged bean sprout extracts at different concentrations, which were 5%, 10%, 15% and 20% applied in the MS media. Data were collected based on number of leaves, length of leaves and length of roots. In this study, the positive control treatment with MS + 5 mg/L BAP media recorded the highest mean value in the number of leaves, length of leaves, and length of roots with values of 12.5 ± 0.59, 5.69 ± 0.13cm, and 6.45 ± 0.36cm respectively followed by MS media with centrifuged bean sprout extract. From the observation, the bean sprout has the potential to use as an additive for Musa acuminata media to substitute synthetic hormones such as BAP.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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