Implementation of real-time image processing on bacterial cellulose formation using soybean-boiled wastewater with the variation of carbon sources during fermentation

Department of Agroindustrial Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Flora Street Number 1, Yogyakarta 55281, Indonesia

^* Corresponding author: ari-nugroho@ugm.ac.id

Abstract

Bacterial cellulose (BC) is produced by aerobic bacteria through oxidative fermentation in synthetic and non-synthetic mediums. Several mediums reported to be used as BC formation mediums are coconut water and soybean-boiled wastewater. Carbon sources are needed to optimize the BC formation process. Recent study has implemented a real-time image processing approach for monitoring BC formation. This study aimed to investigate the correlation between variables that influence the fermentation and to determine the kinetic model of BC formation using an image processing approach with the variation of carbon sources during the fermentation. The results showed that the correlation between fermentation time and thickness had the highest percentage for glucose, sucrose, and mannitol mediums. The kinetic observation of BC formation in the medium using glucose, sucrose, and mannitol followed the Gompertz model equation, with the medium using sucrose having the fastest rate of increase at the 44^th hour, followed by the medium using mannitol at the 112^th hour, and the medium using glucose at the 149^th hour.