Determination of optimal thickness level of Komagataeibacter xylinus bacterial cellulose based on media composition of fructose, ammonium sulfate, and soybean boiling wastewater using Taguchi method

Department of Agro-industrial Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jalan Flora 1 Bulaksumur, Yogyakarta 55281, Indonesia

^* Corresponding author: ari-nugroho@ugm.ac.id

Abstract

Research has focused on finding the optimal media composition to enhance the production of Komagataeibacter xylinus bacterial cellulose using the 3-factor and 2-level Taguchi method and determining the kinetic model for bacterial cellulose formation in the optimal media composition. The factors considered were fructose, ammonium sulfate, and soybean boiling waste. The results showed that the optimal composition for stimulating the formation of K. xylinus bacterial cellulose is fructose 10% (w/v), ammonium sulfate 0.24% (w/v), and soybean boiling waste 20% (v/v). Moreover, the Gompertz curve model was employed to elucidate the relationship between time and thickness during fermentation, yielding mathematical equations. The kinetic model of the optimal composition used the Gompertz model, resulting in the equation y = 4893377e^{-4843515e-0.00031789x}.