Analysis Resistance of Malathion and Cypermethrin Insecticide on Aedes aegypti (Linnaeus, 1762) from Kaliwungu Kudus and Kotagede Yogyakarta Using CDC Bottle Bioassay

¹ Laboratory Animal Systematic, Faculty of Biology Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, Yogyakarta. Indonesia 55281
² Laboratory of Vector and Disease-carrying animals, Center for Environmental Health and Disease Control Techniques, Yogyakarta

^* Corresponding author: upiekastuti@ugm.ac.id

Abstract

Aedes aegypti is a primary vector of Dengue infection and is frequently found near to human dwellings. Indonesia is a tropical country with environmentally suitable for mosquito breeding. Insecticides are commonly used to control mosquito population, however long and continuous use it will create resistance. This study aimed to determine the resistance of Aedes aegypti populations from Kaliwungu District, Kudus Regency and Kotagede District, Yogyakarta City to malathion and cypermethrin using CDC Bottle Bioassay. Ovitraps were placed in 50 houses of each location, and the eggs were brought to the laboratory for rearing until adulthood. Fifteen of female mosquitoes were used as test for each concentration of malathion and cypermethrin. Results showed that Ae. aegypti mosquitoes from Kaliwungu and Kotagede were resistant to malathion at the diagnostic dose of 1x. However, at the 2x diagnostic test, mosquito from Kaliwungu showed tolerant, while mosquito from Kotagede was susceptible to malathion. The cypermethrin test showed that both mosquito samples were resistant and susceptible at the diagnostic dose of 2x and 5x respectively.