Characteristics of collagen from swim bladder of catfish (Pangasius sp.) under different ultrasound exposure times

Department of Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, IPB University, Jl Agatis Kampus IPB Dramaga, Bogor, West Java 16680, Indonesia

^* Corresponding author: mnurilmala@apps.ipb.ac.id

Abstract

The swim bladder is fishery by-product whose main components are collagen protein. Collagen is the main structural component of connective tissue. This study aimed to determine the chemical composition, structure of catfish swim bladder tissue, extraction process, and collagen hydrolysis of catfish swim bladder with different sonication times (0, 10, 15, and 20 minutes). The swim bladder is composed of wavy fibers, branches and spaces between fibers. The best treatment for catfish swim bladder collagen was determined using ANOVA and Duncan's test. The best quality collagen hydrolysate was obtained from a sonication treatment time of 15 minutes. Collagen contained α₁, α₂, and β chains. Collagen hydrolysate contained functional groups with peak absorption of amide A, amide B, amide I, amide II, and amide III, with a pH value of 6.67±0.15, viscosity value of 4.00±1.92, and antioxidant activity with an IC50 value of 44.09±0.48.