Characterization and application of gelatin from red snapper skin for making wound healing membranes

¹ Department of Fisheries Science, Faculty of Agriculture, Universitas Gadjah Mada, Indonesia
² Department of Biomedical, Faculty of Dental & Oral Medicine, Universitas Gadjah Mada, Indonesia

^* Corresponding author: lsahubawa@ugm.ac.id

Abstract

This study used the electrospinning method to process and characterize gelatin from red snapper skin for use as a raw material in biomedical membrane preparations. The research design used treatments involving different "ratios of fish gelatin to pullulan": 50% bovine gelatin: 50% pullulan (p0/control), 25% fish gelatin: 75% pullulan (p1), 50% fish gelatin: 50% pullulan (p2), and 75% fish gelatin: 25% pullulan (p3). The profile of gelatin from red snapper skin includes yield (12.27±3.37%), pH (5.05±0.06), moisture (3.45±0.20%), ash (1.46±0.44%), protein (53.40±3.15%), viscosity (9.24cP), and gel strength (99.37 bloom). Based on light microscopy observations, the percentage of nanofiber membrane threads formed (from the largest size/highest quality to the smallest size/lowest quality) was produced by treatments p0, p1, p2, and p3. SEM observations revealed that the size and shape of the threads in treatments p1 and p0 were relatively similar (<1 μm). FTIR analysis indicated that p0 and p1 have the same four functional groups (aliphatic phosphate, aliphatic secondary amine, monosubstituted alkyl, and aliphatic hydrocarbons). The p0 sample was superior because it also had one additional group detected: phenol. In the solubility test, p1 dissolved more slowly, taking 9.33±3.21 seconds, whereas p0 dissolved in 4.67±0.58 seconds.