Issue |
BIO Web Conf.
Volume 154, 2025
15th International Conference on Global Resource Conservation (ICGRC 2024) in conjunction with the 1st International Conference on Jamu and Alternative Medicine (ICJAM 2024)
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Article Number | 03008 | |
Number of page(s) | 12 | |
Section | Jamu and Alternative Medicine | |
DOI | https://doi.org/10.1051/bioconf/202515403008 | |
Published online | 28 January 2025 |
Vitamin D and Tinospora cordifolia modulate TLR3 and TLR4 pathways, reduce inflammation, and maintain antimicrobial peptide levels in infected mice
1 Student of the Doctoral Program, Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Indonesia
2 Department of Pharmacy, Faculty of Health Sciences, University of Muhammadiyah Malang, Indonesia
3 Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Indonesia
4 Department of Medicine Education, Faculty of Medicine, University of Muhammadiyah Malang, Indonesia
* Corresponding author: immunobiology@ub.ac.id
The activation of Toll-Like Receptor-3 (TLR3) and Toll-Like Receptor-4 (TLR4) signalling pathways is a regular pathway for immune system activation during infection. This study aimed to investigate the effects of vitamin D (VD) and Tinospora cordifolia ethanol extract (TC) on TLR3 and TLR4 receptor protein expression, proinflammatory cytokine (IL1 and IL-6) production, and antimicrobial peptide cathelicidin (CAP) production in CD11b+ cells of mice infected with Escherichia coli. The treatments consisted of administration of VD (0.325 µg/kg bw), TC (100 mg/kg bw), and a combination of both in the same dose for 28 days, followed by induction of E. coli infection on day 29. The flow cytometry method was analyzed of TLR3, TLR4, IL-1, IL-6, and CAP expression in CD11b+ cells of experimental animals. The following measurement results were compared with healthy controls and infected animals with the significance of differences between treatments analyzed by One-way ANOVA with p < 0.05. The results showed that administering VD, TC, and a combination of both reduced the expression of TLR3, TLR4, and IL-1 compared to treating infected animals. The combination treatment of VD + TC increased CAP production more than all other treatments. This significant finding suggests that the combination of VD + TC has the potential to control inflammation without disrupting the body’s defence mechanisms against infection, providing valuable insights for the field of immunology.
© The Authors, published by EDP Sciences, 2025
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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