Open Access
BIO Web Conf.
Volume 24, 2020
International Conferences “Plant Diversity: Status, Trends, Conservation Concept” 2020
Article Number 00054
Number of page(s) 4
Published online 21 September 2020

© The Authors, published by EDP Sciences, 2020

Licence Creative CommonsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

1 Introduction

Gooseberry (Grossularia Mill) is one of the most common berry crops in Siberia. Among its advantages are high productivity, excellent taste and commodity qualities of berries, valuable biochemical composition of fruits [1, 2, 3]. But despite its advantages, gooseberry are characterized by a weak development of plant phytomass when propagated by stem cuttings, and as a result, obtaining low-quality planting material, so it is necessary to look for ways to improve the technology of its propagation [4]. The purpose of the research is to determine the content of macro-and microelements in leaves and the area of the assimilation surface during the cultivation the Grossularia Mill planting material depending on different backgrounds of non-root fertilizing.

2 Materials and Methods

The experiment was conducted in 2018-2019 at the green cuttings section of LLC «Garden Center of the Agrarian University)). Cuttings were rooted in conditions of small-drop irrigation in a greenhouse with a translucent polycarbonate fence. A mixture of peat+sand+soil (leached chernozem) in a volume ratio of 1:1:1 was used as a substrate. The planting scheme of cuttings is 5 x 5 cm. In 2019, rooted cuttings for harvesting were planted in open ground on the phyto section of the Department of Crop Production, Selection and Seed Production. The planting scheme is 40 x 40 cm. The experience is repeated three times, the placement is systematic. Variants of the experiment included non-root treatment (spraying) with water solutions of macro-and microelements in chelated form: 1) control (water spraying); 2) Zn 1% + Cu 1% + 6 trace elements (Mn-0.018%, Fe-0.02%, Mo-0.004%, Co-0.00018%, B-0.0014%, Mg-0.0025%). 3) N-1,6% + 8 trace elements (Fe-0,4%, Cu-0,12%, B-0,028%, Mn-0,36%, Zn-0,09%, Mg-0,05%, Mo-0,08%, Co-0,016%) – these concentrations of macro-and microelements are part of preparations prepared by manufacturers (LLC “NVP “Bashinkom”). Treatment with solutions of macro-and microelements was carried out using an aerosol sprayer with a frequency of 1 time in 2 weeks. In the 1st year of research (2018), spraying was carried out 5 times, in the 2nd year (2019) 6 times. Treatment with solutions was carried out in the early morning hours – in the 1st year of studies, when the cuttings were rooted in the green cuttings area, fine-drip watering after spraying was turned off for 1.5 hours. The object of research is the gooseberry variety – African. Green cuttings were performed according to the generally accepted method [5]. The state of the photosynthetic apparatus of the leaf is described by a number of indicators. In our research, the size of the leaf area was taken into account. The area of the leaf blade was determined for the second year of cuttings growth in open ground after the end of plant growth was completed by the pallete method [6]. Mobile forms of macro- and microelements were determined on the atomic absorption analyzer PinAAcle 900T [7] at the Research Testing Center of the FSBEI HE Krasnoyarsk State Agrarian University. Mathematical processing of the research results was performed by analysis of variance using the MS Excel computer program.

2 Results and discussion

The processes of growth and development in plants are closely interconnected. The term “growth” is understood, as a rule, quantitative changes that occur during development. Leaf area is a phenotypic manifestation of plant growth in the form of a growth parameter, which is formed due to the interaction of the genotype and the environmental factor [8]. The area of the assimilation surface characterizes the strength of plant growth. According to the values of this parameter, we can judge the role of treatment with solutions of m cro-and microelements in changing the area of the leaf blade. Gooseberry plants in the variant using exogenous top-dressing Zn 1% + Cu 1% + 6 microelements showed an increase in the area of the leaf blade-12.9 cm2, which is higher than the control variant by 3.4 cm2, the result was statistically confirmed (SSD05=2.1) (Fig. 1).

The background of N-1.6% + 8 microelements contributed to a decrease in the area of assimilation surface in gooseberry plants, the indicator was lower than the control variant by 1.1 cm2 (8.4 cm2) and below the variant Zn 1% + Cu 1% + 6 microelements by 4, 5 cm2 (8.4 cm2), which is confirmed statistically (SSD05 = 1.6).

The distribution of macro-and microelement composition of leaves differed depending on the background of non-root treatments. The level of accumulation of copper, zinc, manganese and cobalt in the variants with non-root treatments exceeded the control (table 1).

Copper and zinc are biogenic nutrients, but in excess doses they are toxic [9]. The content of these elements in the leaves ranged from 9.6 to 11.7 mg / kg, but the results were not statistically confirmed (p> 0.05).

The concentration in the Fe and Mg leaves on the Zn treatment plot of 1% Cu of 1% 6 microelements is lower than in the control variant, despite this, the area of the assimilation surface in this variant exceeds the control.

Cobalt has a positive effect on plant growth, participates in carbohydrate and mineral metabolism, the synthesis of chlorophyll in leaves, increases the intensity of respiration and the content of ascorbic acid in plants. Accumulation of Co in gooseberry leaves ranged from 1.6-2.0 mg/kg, on versions using non-neural feeding, the cobalt content increased to 2.0 mg/kg.

The level of magnesium content on the studied backgrounds was higher in comparison with the control (115.2-128.3 mg / kg). Mg is involved in the production of energy by plant organisms, with a lack of magnesium, the root system is poorly developed.

thumbnail Fig. 1

Gooseberry leaf surface area, 2019, cm2

Table 1

Influence of non-root treatments on the accumulation of mobile forms of macro-and microelements in gooseberry leaves, August, 2019

4 Conclusion

As a result of research, it was found that non-root feeding of Zn 1% + Cu 1% + 6 microelements of gooseberries when growing planting material by green cutting increases the level of micronutrient accumulation (Zn, Co, Mn) in leaves and activates growth processes – contributes to the development of leaf apparatus.


  • N.A. Mistratova, A.A. Kolichenko, E.A. Savinich, Bul. of the Kras. St. Agr. Univ., 5, 59-65 (2018) [Google Scholar]
  • V.N. Sorokopudov, V.A. Melkumova, O.A. Sorokopudova Gooseberry in Siberia, 25 (Novosibirsk book publishing house, Novosibirsk, 1999) [Google Scholar]
  • V.L. Bopp., E.M. Kuzmina, N.A. Mistratova Fruit-growing of Siberia, 263-275 (Krasnoyarsk, KrasSAU, 2020) [Google Scholar]
  • N.A. Mistratova, D.V. Gotkin., E.V. Bryukhanov, D.S. Romanovsky, Bul. of the Bur. Agric. Acad. named after V.R. Filippov, 4, 111-117 (2019) [Google Scholar]
  • M.T. Tarasenko Green cuttings of garden and forest crops, 15-200 (Publishing house of the Moscow Agricultural Academy, Moscow, 1991) [Google Scholar]
  • V.F. Moiseichenko Methodology of experimental work in fruit growing and vegetable growing, 118-119 (Kiev, High school, 1988) [Google Scholar]
  • Guidelines for the determination of heavy metals in agricultural soils and crop products (1992) [Google Scholar]
  • T.V. Yanchuk, E.N. Sedov, Mod. Gard., 1, 12-17 (2017) Electronic journal [Google Scholar]
  • L.I. Leontieva, Mod. Gard., 2, 84-93 (2018) [Google Scholar]

All Tables

Table 1

Influence of non-root treatments on the accumulation of mobile forms of macro-and microelements in gooseberry leaves, August, 2019

All Figures

thumbnail Fig. 1

Gooseberry leaf surface area, 2019, cm2

In the text

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