The effect of a candidate feed additive derived from the essential oils of Pinus merkusii (jungh. & de vriese) and Melaleuca leucadendra (l.) on the kinetics of gas production and methane emitted during in-vitro ruminal fermentation

Open Access

Table 1.

Cumulative gas and methane production from in vitro rumen fermentation added with three blends of essential oil.

BEO	Control					BEO3
Doses (µl/l)	0	100	200	100	200	100
Vol Gas (ml/200mg DM)	37,83±2,47	39,17 ±1,89 36,67 ±5,03 38,67±2,36 38,67±2,08				36,67 ±2,08
CH4 (ml/g DOM)	20,16^ab±2,66	22,04^ab ±2,38 14,78^a ±4,72 26,27^b±7,41 20,01^ab±0,92				15,76^a ±2,47

^ab

different superscript in the same row indicated significant difference (P<0.05)

BEO: blend essential oil of pine (Pinus merkusii) and eucalyptus (Melaleuca leucadendra in ratio BEO1: 75:25, BEO2: 50:50, and BEO3: 25:75

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