Protamine-2 protein abundance in Ongole Grade mature spermatozoa is unrelated to semen quality and DNA fragmentation

Open Access

Issue		BIO Web Conf. Volume 162, 2025 Wildlife-Endangered Species Conservation and Animal Reproduction (WECARe) International Conference 2024


Article Number		00006
Number of page(s)		10
DOI		https://doi.org/10.1051/bioconf/202516200006
Published online		28 February 2025

E. Romjali, Local beef cattle breeding program in Indonesia, Wartazoa. 28, 190–210 (2018). http://dx.doi.org/10.14334/wartazoa.v28i4.1813 [Google Scholar]
G. Sitanggang, Environmental effects and repeatability estimates on semen quality in Ongole Grade bulls. JIPI. 23, 88–92 (2018). http://dx.doi.org/10.18343/jipi.23.2.88 [CrossRef] [Google Scholar]
P. Santolaria, J.P. Rickard, R. Pérez-Pe, Understanding sperm quality for improved reproductive performance. Biology. 12, 980 (2023). https://doi.org/10.3390/biology12070980 [CrossRef] [PubMed] [Google Scholar]
B.M. Tanga, A.Y. Qamar, S. Raza, S. Bang, X. Fang, K. Yoon, J. Cho, Semen evaluation: methodological advancements in sperm quality-specific fertility assessment A review. Animal bioscience. 34, 1253–1270 (2021). https://doi.org/10.5713/ab.21.0072 [CrossRef] [PubMed] [Google Scholar]
M. Hirose, A. Honda, H. Fulka, M. Tamura-Nakano, S. Matoba, T. Tomishima, K. Mochida, A. Hasega, K. Nagashima, K. Inoue, M. Ohtsuka, T. Baba, R. Yanagimachi, A. Ogura, Acrosin is essential for sperm penetration through the zona pellucida in hamsters. Proc Natl Acad Sci U S A. 117, 2513–2518 (2020). https://doi.org/10.1073/pnas.1917595117 [CrossRef] [PubMed] [Google Scholar]
R.G. Saacke. Sperm morphology: its relevance to compensable and uncompensable traits in semen. Theriogenology. 70, 473–478 (2018). https://doi.org/10.1016/j.theriogenology.2008.04.012 [CrossRef] [PubMed] [Google Scholar]
M. Kleshchev, A. Osadchuk, L. Osadchuk, Impaired semen quality, an increase of sperm morphological defects and DNA fragmentation associated with environmental pollution in urban population of young men from Western Siberia, Russia. PLOS ONE. 16, e0258900 (2021). https://doi.org/10.1371/journal.pone.0258900 [CrossRef] [PubMed] [Google Scholar]
L. Simon, J. Castillo, R. Oliva, S.E.M. Lewis, Relationships between human sperm protamines, DNA damage, and assisted reproduction outcomes. Reprod Biomed Online. 23, 724–34 (2011). https://doi.org/10.1016/j.rbmo.2011.08.010 [CrossRef] [PubMed] [Google Scholar]
T. Wang, H. Gao, W. Li, C. Liu, Essential role of histone replacement and modifications in male fertility. Front Genet. 10, 962 (2019). https://doi.org/10.3389/fgene.2019.00962 [CrossRef] [PubMed] [Google Scholar]
R. Olivia, Protamines and male infertility. Hum Reprod Update. 12, 417–35 (2006). https://doi.org/10.1093/humupd/dml009 [Google Scholar]
R. Suganuma, R. Yanagimachi, M.L. Meistrich, Decline in fertility of mouse sperm with abnormal chromatin during epididymal passage as revealed by ICSI. Hum Reprod. 20, 3101–3108 (2005). https://doi.org/10.1093/humrep/dei169 [Google Scholar]
W.M. Maier, G. Nussbaum, L. Domenjoud, U. Klemm, W. Engel, The lack of protamine 2 (PT 2) in boar and bull spermatozoa is due to mutations within P2 gene. Nucleic Acids Res. 18, 1249–1254 (1990). https://doi.org/10.1093/nar/18.5.1249 [CrossRef] [PubMed] [Google Scholar]
I. Ganguly, G.K. Gaur, S. Kumar, D.K. Mandal, M. Kumar, U. Singh, S. Kumar, A. Sharma, Differential expression of protamine 1 and 2 genes in mature spermatozoa of normal and motility impaired semen producing crossbred Frieswal (HF x Sahiwal) bulls. Res Vet Sci. 94, 245–62 (2013). https://doi.org/10.1016/j.rvsc.2012.09.001 [Google Scholar]
Y.Q. Lv, X. Chen, D. Xu, X.T. Luo, M.M. Cheng, Y.Y. Zhang, X.L. Qu, Y. Jin Y, Effects of crocin on frozen-thawed sperm apoptosis, protamine expression and membrane lipid oxidation in Yanbian yellow cattle. Reprod Domest Anim. 55, 10111020 (2020). https://doi.org/10.1111/rda.13744 [Google Scholar]
H. Rodríguez-Martínez, Evaluation of frozen semen: traditional and new approaches. In: Topics in Bull Fertility (Chenoweth PJ, International Veterinary Information Service, Ithaca, New York, USA, 2000). [Google Scholar]
B.P. Pardede, A. Kusumawati, M. Pangestu, B. Purwantara, Bovine sperm HSP-70 molecules: a potential cryo-tolerance marker associated with semen quality and fertility rate. Front Vet Sci. 10, 1167594 (2023). https://doi.org/10.3389/fvets.2023.1167594 [CrossRef] [PubMed] [Google Scholar]
B.P. Pardede, N.W.K. Karja, S. Said, E.M. Kaiin, M. Agil, C. Sumantri, B. Purwantara, I. Supriatna, Bovine nucleoprotein transitions genes and protein abundance as valuable markers of sperm motility and the correlation with fertility. Theriogenology. 215, 86–94 (2024). https://doi.org/10.1016/j.theriogenology.2023.11.015 [CrossRef] [PubMed] [Google Scholar]
A. Kusumawati, F.A. Satrio, R. Indriastuti, Z.N.A. Rosyada, B.P. Pardede, M. Agil, B. Purwantara, Sperm head morphology alterations associated with chromatin instability and lack of protamine abundance in frozen-thawed sperm of indonesian local bulls. Animals. 13, 2433 (2023). https://doi.org/10.3390/ani13152433 [CrossRef] [PubMed] [Google Scholar]
R. Puglisi, A. Pozzi, L. Foglio, M. Spano, P. Eleuteri, M.G. Grollino, G. Bongioni, A. Galli, The usefulness of combining traditional sperm assessments with in vitro heterospermic insemination to identify bulls of low fertility as estimated in vivo. Anim Reprod Sci. 132, 17–28 (2012). https://doi.org/10.1016/j.anireprosci.2012.04.006 [CrossRef] [PubMed] [Google Scholar]
BSN (Badan Standarisasi Nasional), SNI (Standar Nasional Indonesia) Semen Beku Bagian 1: Sapi SNI 4869.1-2017 (Badan Standarisasi Nasional, Jakarta, Indonesia, 2017). [Google Scholar]
J.M. Morrell, H. Rodriguez-Martinez, Biomimetic techniques for improving sperm quality in animal breeding: a review. J Androl. 1, 1–9 (2009). [Google Scholar]
A.R. Reveco, J.L. Hernández, P. Aros. Long-term storing frozen semen at −196 °C does not affect the post-thaw sperm quality of bull semen in cryopreservation in eukaryotes. IntechOpen. p91101 (2016). [Google Scholar]
India Agriculture Ministry, Compendium of minimum standards of protocol and standard operating procedures for bovine breeding (Department of Animal Husbandry, Dairying, and Fisheries, India, 2014). [Google Scholar]
G.K. Patel, N. Haque, M. Madhavatar, A.K. Chaudhari, D.K. Patel, N. Bhalakiya, N. Jamnesha, P. Patel, R. Kumar, Artificial insemination: A tool to improve livestock productivity. J Pharm and Phytoch. 1, 307–313 (2017). [Google Scholar]
A. Khezri, B. Narud, E. Stenseth, A. Johannisson, F.D. Myromslien, A.H. Gaustad, R.C. Wilson, R. Lyle, J.M. Morrell, E. Kommisrud, R. Ahmad, DNA methylation patterns vary in boar sperm cells with different levels of DNA fragmentation. BMC Genomics. 20, 897 (2019). https://doi.org/10.1186/s12864-019-6307-8 [CrossRef] [PubMed] [Google Scholar]
J.M. D’Occhio, K.J. Hengstberger, D. Tutt, R.G. Holroyd, G. Fordyce, G.B. BoeHansen, S.D. Johnston, Sperm chromatin in beef bulls in tropical environments. Theriogenology. 79, 946–52 (2013). https://doi.org/10.1016/j.theriogenology.2013.01.012 [CrossRef] [PubMed] [Google Scholar]
A. Champroux, J. Torres-Carreira, P. Gharagozloo, J.R. Drevet, A. Kocer, Mammalian sperm nuclear organization: resiliencies and vulnerabilities. Basic Clin Androl. 26, 17 (2016). https://doi.org/10.1186/s12610-016-0044-5 [CrossRef] [PubMed] [Google Scholar]
L. Moritz, S.B. Schon, M. Rabbani, Y. Sheng, R. Agrawal, J. Glass-Klaiber, C. Sultan, J.M. Camarillo, J. Clements, M.R. Baldwin, A.G. Diehl, A.P. Boyle, P.J. O’Brien, K. Ragunathan, Y.C. Hu, N.L. Kelleher, J. Nandakumar, J.Z. Li, K.E. Orwig, S. Redding, S.S. Hammoud, Sperm chromatin structure and reproductive fitness are altered by substitution of a single amino acid in mouse protamine 1. Nat Struct Mol Biol. 30, 10771091 (2023). https://doi.org/10.1038/s41594-023-01033-4 [CrossRef] [PubMed] [Google Scholar]
T.R.S. Hamilton, R. Simoes, C.M. Mendes, M.D. Goissis, E. Nakajima, E.A.L. Martins, J.A. Visintin, M.E.O.A. Assumpcao, Detection of protamine 2 in bovine spermatozoa and testicles. Andrology. 7, 373–381 (2019). https://doi.org/10.1111/andr.12610 [CrossRef] [PubMed] [Google Scholar]
S. Moghbelinejad, R. Najafipour, A.S. Hashjin, Comparison of protamine 1 to protamine 2 mRNA ratio and ybx2 gene mRNA content in testicular tissue of fertile and azoospermic men. Int J Fertil Steril. 9, 338–45 (2015). https://doi.org/10.22074/ijfs.2015.4549 [PubMed] [Google Scholar]

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