Butyrate and Resveratrol synergize to inhibit colorectal cancer cell proliferation and migration by preventing the nuclear translocation of β-catenin

Open Access

Issue		BIO Web Conf. Volume 187, 2025 2025 Joint Meeting of International Conference of Nutritional Fortification (ISPH-ISNPR 2025)


Article Number		04001
Number of page(s)		11
Section		Cancer Medicine
DOI		https://doi.org/10.1051/bioconf/202518704001
Published online		09 September 2025

F. Bray, M. Laversanne, H. Sung, J. Ferlay, R.L. Siegel, I. Soerjomataram, A. Jemal, Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J. Clin. 74, 229–263 (2024). https://doi.org/10.3322/caac.21834 [Google Scholar]
G. Roshandel, F. Ghasemi-Kebria, R. Malekzadeh, Colorectal cancer: Epidemiology, risk factors, and prevention. Cancers 16, 1530 (2024). https://doi.org/10.3390/cancers16081530 [Google Scholar]
Valle, E. Vilar, S.V. Tavtigian, E.M. Stoffel, Genetic predisposition to colorectal cancer: syndromes, genes, classification of genetic variants and implications for precision medicine. J. Pathol. 247, 574–588 (2019). https://doi.org/10.1002/path.5229 [Google Scholar]
Q. Cao, Y. Tian, Z. Deng, F. Yang, E. Chen, Epigenetic alteration in colorectal cancer: Potential diagnostic and prognostic implications. Int. J. Mol. Sci. 25, 3358 (2024). https://doi.org/10.3390/ijms25063358 [Google Scholar]
E. Dekker, P.J. Tanis, J.L.A. Vleugels, P.M. Kasi, M.B. Wallace, Colorectal cancer. Lancet 394, 1467–1480 (2019). https://doi.org/10.1016/S0140-6736(19)32319-0 [Google Scholar]
L. Zhang, J.W.Shay, Multiple Roles of APC and its Therapeutic Implications in Colorectal Cancer. J Natl Cancer Inst. 109(8), djw332 (2017). https://doi.org/10.1093/jnci/djw332. [Google Scholar]
M.I. Pronobis, N.M. Rusan, M. Peifer, A novel GSK3-regulated APC:Axin interaction regulates Wnt signaling by driving a catalytic cycle of efficient βcatenin destruction. eLife, 4, e08022, (2015). https://doi.org/10.7554/eLife.08022. [Google Scholar]
T. Wang, J. Fu, Y. Huang, C. Fu, Mechanism of APC truncation involved in colorectal cancer tumorigenesis. Oncol. Lett. 29, 2 (2024). https://doi.org/10.3892/ol.2024.14748 [Google Scholar]
J. Fröhlich, K. Rose, A. Hecht, Transcriptional activity mediated by β-CATENIN and TCF/LEF family members is completely dispensable for survival and propagation of multiple human colorectal cancer cell lines. Scientific reports 13, 287 (2023). https://doi.org/10.1038/s41598-022-27261-0 [Google Scholar]
C. Munteanu, B. Schwartz, Interactions between dietary antioxidants, dietary fiber and the gut microbiome: Their putative role in inflammation and cancer. Int. J. Mol. Sci. 25, 8250 (2024). https://doi.org/10.3390/ijms25158250 [Google Scholar]
F. Vernia, S. Longo, G. Stefanelli, A. Viscido, G. Latella, Dietary factors modulating colorectal carcinogenesis. Nutrients 13, 143 (2021). https://doi.org/10.3390/nu13010143 [Google Scholar]
A. Nishida, R. Inoue, O. Inatomi, S. Bamba, Y. Naito, A. Andoh, Gut microbiota in the pathogenesis of inflammatory bowel disease. Clinical journal of gastroenterology, 11(1), 1–10 (2018). https://doi.org/10.1007/s12328-017-0813-5 [Google Scholar]
A. Tripathy, J. Dash, S. Kancharla, P. Kolli, D. Mahajan, S. Senapati, M. K. Jena, Probiotics: A Promising Candidate for Management of Colorectal Cancer. Cancers, 13(13), 3178 (2021). https://doi.org/10.3390/cancers13133178 [Google Scholar]
K. Kaźmierczak-Siedlecka, L. Marano, E. Merola, F. Roviello, K. Połom, Sodium butyrate in both prevention and supportive treatment of colorectal cancer. Front. Cell Infect. Microbiol. 12, 1023806 (2022). https://doi.org/10.3389/fcimb.2022.1023806 [Google Scholar]
P. Liu, Y. Wang, G. Yang, Q. Zhang, L. Meng, Y. Xin, X. Jiang, The role of short-chain fatty acids in intestinal barrier function, inflammation, oxidative stress, and colonic carcinogenesis. Pharmacol. Res. 165, 105420 (2021). https://doi.org/10.1016/j.phrs.2021.105420 [Google Scholar]
L. Vallino, B. Garavaglia, A. Visciglia, A. Amoruso, M. Pane, A. Ferraresi, C. Isidoro, Cellfree Lactiplantibacillus plantarum OC01 supernatant suppresses IL-6-induced proliferation and invasion of human colorectal cancer cells: Effect on β-catenin degradation and induction of autophagy. J. Tradit. Complement Med. 13, 193–206 (2023). https://doi.org/10.1016/j.jtcme.2023.02.001 [Google Scholar]
M. Nemati, G.R. Omrani, B. Ebrahimi, N. Montazeri-Najafabady, The beneficial effects of probiotics via autophagy: A systematic review. Biomed. Res. Int. 2021, 2931580 (2021). https://doi.org/10.1155/2021/2931580 [Google Scholar]
B. Garavaglia, L. Vallino, A. Ferraresi, A. Amoruso, M. Pane, C. Isidoro, Probiotic-derived metabolites from Lactiplantibacillus plantarum OC01 reprogram tumor-associated macrophages to an inflammatory anti-tumoral phenotype: Impact on colorectal cancer cell proliferation and migration. Biomedicines 13, 339 (2025). https://doi.org/10.3390/biomedicines13020339 [Google Scholar]
B. Garavaglia, L. Vallino, A. Ferraresi, A. Esposito, A. Salwa, C. Vidoni, S. Gentilli, C. Isidoro, Butyrate inhibits colorectal cancer cell proliferation through autophagy degradation of β-catenin regardless of APC and β-catenin mutational status. Biomedicines 10, 1131 (2022). https://doi.org/10.3390/biomedicines10051131 [Google Scholar]
Rudzińska A, Juchaniuk P, Oberda J, Wiśniewska J, Wojdan W, Szklener K, Mańdziuk S. Phytochemicals in Cancer Treatment and Cancer Prevention-Review on Epidemiological Data and Clinical Trials. Nutrients. 15(8):1896. (2023). https://doi.org/10.3390/nu15081896. [Google Scholar]
A. Rudzińska, P. Juchaniuk, J. Oberda, J. Wiśniewska, W. Wojdan, K. Szklener, S. Mańdziuk, Phytochemicals in cancer treatment and cancer prevention—review on epidemiological data and clinical trials. Nutrients 15, 1896 (2023). https://doi.org/10.3390/nu15081896 [Google Scholar]
M. Koushki, N. Amiri-Dashatan, N. Ahmadi, H.A. Abbaszadeh, M. Rezaei-Tavirani, Resveratrol: A miraculous natural compound for diseases treatment. Food Science & Nutrition 6(8):24732490 (2018). https://doi.org/10.1002/fsn3.855. [Google Scholar]
M. Honari, R. Shafabakhsh, R.J. Reiter, M. Hamed, Z. Asemi, Resveratrol is a promising agent for colorectal cancer prevention and treatment: focus on molecular mechanisms. Cancer Cell International 19, 180 (2019). https://doi.org/10.1186/s12935-019-0906-y [Google Scholar]
Z. Zhang, Z. Liu, J. Chen, J. Yi, J. Cheng, W. Dun, , H. Wei, H. Resveratrol induces autophagic apoptosis via the lysosomal cathepsin D pathway in human drug-resistant K562/ADM leukemia cells. Experimental and therapeutic medicine, 15(3), 3012–3019 (2018). https://doi.org/10.3892/etm.2018.5742 [Google Scholar]
H. Su, F. Yang, Q. Wang, Q. Shen, J. Huang, C. Peng, Y. et al, VPS34 Acetylation Controls Its Lipid Kinase Activity and the Initiation of Canonical and Non-canonical Autophagy. Molecular cell, 67(6), 907–921.e7 (2017). https://doi.org/10.1016/j.molcel.2017.07.024 [Google Scholar]
M. J. Anwar, A. Altaf, M. Imran, M. Amir, S. A. Alsagaby, W.A Abdulmonem, et al, Anti-cancer perspectives of resveratrol: a comprehensive review. Food and Agricultural Immunology, 34(1). (2023). https://doi.org/10.1080/09540105.2023.2265686 [Google Scholar]
H. Wang, H. Zhang, L. Tang, H. Chen, C. Wu, M. Zhao, Y. Yang, X. Chen, G. Liu, Resveratrol inhibits TGF-β1-induced epithelial-tomesenchymal transition and suppresses lung cancer invasion and metastasis, Toxicology, 303, 139–146 (2013). https://doi.org/10.1016/j.tox.2012.09.017 [Google Scholar]
M. Yunika, S. Takuya, Resveratrol enhances intestinal barrier function byameliorating barrier disruption in Caca-e cell monolayers, Journal of Funcitonal Foods, 51, 39–46. (2018). https://doi.org/10.1016/j.jff.2018.10.009 [Google Scholar]
F. Yu, C. Yu, F. Li, Y. Zuo, Y. Wang, L. Yao, C. Wu, C. Wang, L. Ye, Wnt/β-catenin signaling in cancers and targeted therapies, Signal Transduct. Target Ther., 6(1), 307 (2021). https://doi.org/10.1038/s41392-021-00701-5 [Google Scholar]
R. Ahmad, J.K. Singh, A. Wunnava, O. Al-Obeed, M. Abdulla, S.K. Srivastava, Emerging trends in colorectal cancer: Dysregulated signaling pathways (Review), Int. J. Mol. Med., 47(3), 14 (2021). https://doi.org/10.3892/ijmm.2021.4847 [Google Scholar]
R. Divella, A. Daniele, E. Savino, A. Paradiso, Anticancer effects of nutraceuticals in the Mediterranean diet: An epigenetic diet model, Cancer Genomics Proteomics, 17(4), 335–350 (2020). https://doi.org/10.21873/cgp.20193 [Google Scholar]
J. Liu, Q. Xiao, J. Xiao, C. Niu, Y. Li, X. Zhang, Z. Zhou, G. Shu, G. Yin, Wnt/β-catenin signalling: function, biological mechanisms, and therapeutic opportunities, Signal Transduction and Targeted Therapy, 7(1), 3 (2022). https://doi.org/10.1038/s41392-021-00762-6 [Google Scholar]
C.Y. Loh, J. Y. Chai, T. F. Tang, W.F. Wong, G. Sethi, M. K. Shanmugam, et al, The E-Cadherin and N-Cadherin Switch in Epithelial-to-Mesenchymal Transition: Signaling, Therapeutic Implications, and Challenges. Cells, 8(10), 1118. (2019). https://doi.org/10.3390/cells8101118. [Google Scholar]
O. Burgy, M. Königshoff, The WNT signaling pathways in wound healing and fibrosis, Matrix Biol., 68-69, 67–80 (2018). https://doi.org/10.1016/j.matbio.2018.03.017 [Google Scholar]
Thompson, E.W., Redfern, A.D., Brabletz, S. et al. EMT and cancer: what clinicians should know. Nat Rev Clin Oncol (2025). https://doi.org/10.1038/s41571-025-01058-2 [Google Scholar]
T. Zhan, N. Rindtorff, M. Boutros, Wnt signaling in cancer. Oncogene, 36(11), 1461–1473, (2017). https://doi.org/10.1038/onc.2016.304. [Google Scholar]
S. Shang, F. Hua, Z. Hu, The regulation of β-catenin activity and function in cancer: therapeutic opportunities. Oncotarget, 8(20), 33972–33989, (2017). https://doi.org/10.18632/oncotarget.15687. [Google Scholar]

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