| Issue |
BIO Web Conf.
Volume 8, 2017
2016 International Conference on Medicine Sciences and Bioengineering (ICMSB2016)
|
|
|---|---|---|
| Article Number | 03004 | |
| Number of page(s) | 8 | |
| Section | Session III: Biomedical Engineering | |
| DOI | https://doi.org/10.1051/bioconf/20170803004 | |
| Published online | 11 January 2017 | |
Plant regeneration and Agrobacterium-mediated transformation of Vacuolar H+-ATPase c Subunit Gene in hybrid poplar populus davidiana Dode × P. bollena Lauche
1 College of Accountancy, Harbin University of Commerce, Harbin, 150028, China
2 College of Food Science and Engineering, Harbin University of Commerce, Harbin, 150076, China
a Corresponding author: hb790118@163.com
An efficient regeneration and transformation system was developed for hybrid poplar populus davidiana Dode × P. bollena Lauche. Several factors, such as a competent regeneration protocol, antibiotics, bacterial concentration, infection time have been shown to affect transformation efficiency. It is the first report describing an efficient protocol for Vacuolar H+-ATPase c Subunit Gene from salt tolerant plant Puccinellia chinampoensis, which transformed into populus. Stable transgene integration was confirmed by the expression of GFP fusion proteins in leaves, stem and root for populus.
© The Authors, published by EDP Sciences, 2017
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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