BIO Web Conf.
Volume 8, 20172016 International Conference on Medicine Sciences and Bioengineering (ICMSB2016)
|Number of page(s)||7|
|Section||Session III: Biomedical Engineering|
|Published online||11 January 2017|
School of Life Science, Jiangxi Science & Technology Normal University, Nanchang, China
a Corresponding author: Zengtx001@aliyun.com
Establishment of insulin resistance model of HepG2 cells in vitro is aimed at studying puerarin responsible for the hypoglycemic effect. HepG2 cells induced by palmitate and glucosamine were used to establish the model of insulin resistance. Simultaneously, the effect of the two inducing methods was compared. From the test results of MTT(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) and glucose uptake, it was significantly found insulin resistance of HepG2 cells when it was treated with palmitate under the concentration of 0.125mM. The hypoglycemic effect of puerarin testing by insulin resistance model of HepG2 cells indicated that HepG2 cells induced by low concentration of Palmitate can be used to produce stable and reliable insulin resistance. The establishment of insulin resistance model have a certain value for in vitro screening hypoglycaemic herbal medicine.
© The Authors, published by EDP Sciences, 2017
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