Effect of basal medium on growth and polyphenols accumulation by Gardenia jasminoides Ellis cell suspension

Laboratory of Cell Biosystems, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Plovdiv, Bulgaria

^* Corresponding author: gergana.shk@gmail.com

Abstract

The goal of this study was to analyze the effect of different medium bases on accumulation of biomass and secondary metabolites production by Gardenia jasminoides Ellis. cell suspension culture. The result revealed that different media have significant effect on biomass accumulation and production of polyphenol compounds. HPLC analyses showed that the higher polyphenol content was achieved when the cells were cultivated on McCOWN Woody plant Medium (WP) media for 14 days on darkness, at 24°C. At this conditions, the cells produced maximal amounts of catechin (24.44±2.46 µg/g DW), chlorogenic acid (20.26 ±3.68 µg/g DW), epicatechin (70.44±7.46 µg/g DW) and rutin (45.90±0.26 µg/g DW). Both MS and WP media were optimal for biomass accumulation. When cultivated on these media, Gardenia cells accumulated maximal amount of dry biomass ADB = 0.99±0.1g/100ml and ADB = 0.93±0.04 g/100ml for МS and WP, respectively. The corresponding growth indexes were GIDW = 1.68±0.2 on MS and GIDW = 1.58±0.07 on WP medium. The reported results are the first step of future optimization of the nutrient medium composition and cultivation conditions essential for the scale -up of cultivation process of G. jasminoides cell suspension culture for mass production of polyphenolic compounds.