Open Access
Table 1.
Composition of modified Murashige-Skoog nutrient media
| Components of the medium | MS1 | MS2 | MS3 | MS4 | MS5 | MS6 | MS7 | MS8 |
|---|---|---|---|---|---|---|---|---|
| Volume per 1 L | ||||||||
| Macrosalt stock solutions, ml | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
| Microsalt stock solutions, ml | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Fe‐chelate, ml | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| CaCl2, ml | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| Vitamins: PP, mg | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| B1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
| B6 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| Mesoinositis, mg | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
| Glycine, ml | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
| α‐Naphthaleneacetic acid (NAA), mg | ‐ | ‐ | ‐ | ‐ | ‐ | 0.5 | 2 | ‐ |
| Indole‐3‐acetic acid (IAA), mg | ‐ | ‐ | 1 | ‐ | 2 | ‐ | 2 | 3 |
| Indole‐3‐butyric acid (IBA), mg | ‐ | ‐ | ‐ | 1 | ‐ | ‐ | ‐ | ‐ |
| Kinetin, mg | ‐ | ‐ | ‐ | ‐ | 0.2 | 0.1 | 1 | 2 |
| 6‐benzylaminopurine (6‐BAP), mg | 1 | 3 | ‐ | ‐ | ‐ | 1 | 1 | ‐ |
| 2,4‐Dichlorophenoxyacetic acid (2,4‐D), mg | ‐ | ‐ | ‐ | ‐ | ‐ | ‐ | 2 | ‐ |
| Sucrose, g | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| Agar‐agar, g | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 |
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