Issue |
BIO Web Conf.
Volume 56, 2023
43rd World Congress of Vine and Wine
|
|
---|---|---|
Article Number | 01027 | |
Number of page(s) | 8 | |
Section | Viticulture | |
DOI | https://doi.org/10.1051/bioconf/20235601027 | |
Published online | 24 February 2023 |
Caracterización genotípica y sanitaria de variedades de vid minoritarias prospectadas en Andalucía, España
Genotypic and sanitary characterization of minority grapevine varieties prospected in Andalusia, Spain
1
IFAPA Centro Rancho de la Merced.
Ctra. Cañada de la Loba PK 3,
1 11471
Jerez de la Frontera (Cádiz), España
2
IFAPA Centro de Cabra. Antigua Ctra.
Cabra-Doña Mencía, Km. 2,
5 14940
Cabra (Córdoba), España
3
IFAPA Centro de Málaga.
Cortijo de la Cruz s/n
29140
Málaga, España
4
IMIDA
Calle Mayor s/n
30150
La Alberca (Murcia), España
Debido a su diversidad y extensión territorial, Andalucía es una región española que alberga numerosas variedades minoritarias, ofreciendo a la vitivinicultura local la posibilidad de diversificar su producción vitivinícola. Desde 2020, se ha realizado la identificación molecular de 98 ejemplares procedentes de seis zonas con tradición vitivinícola: la Alpujarra (9 individuos) y el Altiplano (2) en Granada; Moguer (12) en Huelva; el Valle de los Pedroches (36) y Montilla-Moriles (32) en Córdoba; el Pago Burujena (7)en Cádiz. Se han empleado trece marcadores microsatélites, entre ellos los nueve recomendados por la OIV. Se han obtenido 31 genotipos distintos, 20 se corresponden a perfiles de variedades ya descritas, los otros 11 no se han identificado previamente. Los perfiles se han integrado en el database del banco de germoplasma de vid “Rancho de la Merced”. Mediante análisis de diversidad genética, se ha determinado en cuales grupos eco-geográficos se colocan los nuevos genotipos identificados. Al tratarse de nuevas variedades con un interés potencial en ser autorizadas, se quiere disponer de clones certificables; por ello, se ha analizado la presencia de los virus del enrollado, del entrenudo corto infeccioso y del jaspeado de la vid, mediante test ELISA.
Abstract
Since 2020, molecular identification has been performed for 98 individuals from six traditional vitiviniculture zones: “la Alpujarra” (9 vines) and “el Altiplano” (2), Granada; “Moguer” (12), Huelva; “el Valle de los Pedroches” (36) and “Montilla-Moriles” (32), Cordoba; “el Pago Burujena” (7), Cadiz. Thirteen microsatellites were used, including the nine SSR set recommended by the OIV. Thirty-one different genotypes were obtained, twenty out of the total correspond to already described varieties, while the other eleven ones were not identified previously. Genotypic profiles were integrated in the “Rancho de la Merced” grapevine repository database. Diversity genetic analyses were performed in order to assign unidentified genotypes to eco-geographic grapevine variety groups. In order to accelerate the processes of potential authorization and commercialization of new varieties, the presence of certifiable materials was investigated. Therefore, virus infection test by ELISA were performed for Grapevine Fanleaf Virus (GFLV), Grapevine Leafroll associated Viruses (GLRaVs) and Grapevine Fleck Virus (GFkV).
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0 (http://creativecommons.org/licenses/by/4.0/).
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